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SETSCI - Volume (2018)
ISAS 2018 - Ist International Symposium on Innovative Approaches in Scientific Studies, Kemer-Antalya, Turkey, Apr 11, 2018

The Effects of Epstein Barr Virus Recombinant Proteins EBNA 1 and LMP 1 on The Endoplasmic Reticulum Stress, Apoptosis and Cell Proliferation in Pancreatic Beta Cell Culture (ISAS 2018_27)
Merve Şen1*, Mustafa Altındiş2, Serkan Şen3, Sefa Çelik4, Korhan Altunbaş5, İsmail Hakkı Ciğerci6
1Fethiye Sağlık Hizmetleri Meslek Yüksekokulu,Muğla Sıtkı Koçman Üniversitesi, Muğla, Turkey
2Deparmet of Medical Microbiology, Medicine Faculty, Sakarya University , Sakarya, Turkey
3Fethiye Sağlık Hizmetleri Meslek Yüksekokulu,Muğla Sıtkı Koçman Üniversitesi, Muğla, Turkey
4Department of Medical Biochemistry, Medicine Faculty, Afyon Kocatepe University , Afyon, Turkey
5Departmet of Embriyology and Histology, Veterinary Faculty, Afyon Kocatepe University , Afyon, Turkey
6Departmen of Molecular Biology and Genetic, Art and Science Faculty, Afyon Kocatepe University , Afyon, Turkey
* Corresponding author: mervesen@mu.edu.tr
Published Date: 2018-06-23   |   Page (s): 27-27   |    64     8

ABSTRACT Proliferative activity of Epstein Barr Virus (EBV) proteins occurs via two mediators (Epstein Barr Nuclear Antigen1-EBNA1 and latent membrane Protein1-LMP1). In this study, it is aimed to investigate that recombinant viral EBV protein of pancreatic cell loss those applying to reverse. In addition to that related proteins as well as DNA damage and stress effects on insulin synthesis and release and their contributions were also investigated. Insulin analyses were carried out by ELISA techniques using commercial kit for insulin. Comet assay for DNA damage and on the other hand to ER stress, levels of mRNA expression of the gene of interest were tested. As a result of RT-PCR applications, mRNA expression levels of genes of ATF4, ATF6, PERK, GRP78 and CHOP, which are ER stress markers, orderly increased 10-fold, 19-fold, 15,03-fold, 6,63-fold, and 8,05-fold according to DMSO control group in thapsigargin group. It is observed this increase on the expression of the genes which are ER stress marker is repressed by interest proteins. The conclusion was that EBV recombinant proteins LMP1 and EBNA1 proliferation genes which are responsible for expression levels increased but also ER stress marker gene expression level by suppressing the ER stress-induced beta cell loss in reversing would be advantageous. In this regard, our results, if supported by further studies, the EBV will be possible to use as virotherapic agents in antidiabetic treatment.  
KEYWORDS Epstein Barr Virus, EBNA1, LMP1, Diabetes, Endoplasmic Reticulum Stress
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