The aim of this study was to investigate how resveratrol administration  diabetic elderly female  rats affects the levels of bone tissue SIRT1. The study was performed on female aged rats (16 months old) who were provided by The Experimental Medicine Research and Application Center of Selçuk University.  A total of 24 elderly female rats were divided into 4 groups: Group 1. Control, Group 2. Control + Resveratrol, Group 3. Diabetes, Group 4. Diabetes + Resveratrol. In order to induce diabetes, the animals were intraperitoneally (IP) injected with 40 mg/kg streptozotocin (STZ). The animals were supplemented with 5 mg/kg/day resveratrol IP for 4 weeks.  At the end of the study, SIRT1 protein gene expression  were determined by PCR, on bone tissue samples obtained from the  animals  sacrificed  under general anesthesia. In our study, the highest bone tissue SIRT1 expression values were obtained in the diabetes + resveratrol group (G4) and the lowest bone SIRT 1 levels were found in the diabetes group (G3). Our results suggest that resveratrol supplementation increases bone SIRT1 expression in diabetic elderly female rats

Keywords - old female rats, diabetes mellitus, resveratrol supplementation, SIRT1, bone tissue

[1] O. Tell-Lebanon, P. Rotman-Pikielny. “Osteoporosis and diabetes - in which way are they related?.” Harefuah ;155(11):697-701 2016.
[2] G. Giovos, MP. Yavropoulou, JG. Yovos. “The role of cellular senescence in diabetes mellitus and osteoporosis: molecular pathways and potential interventions.” Hormones (Athens) Nov 8. doi: 10.1007/s42000-019-00132-6, 2019.
[3] Y. Fei, E. Shimizu, MW. McBurney, NC. Partridge. “Sirtuin 1 is a negative regulator of parathyroid hormone stimulation of matrix metalloproteinase 13 expression in osteoblastic cells: role of sirtuin 1 in the action of PTH on osteoblasts.” J Biol Chem ;290(13):8373-8382 2015.
[4] QB. Zhang, W. Cao, YR. Liu, SM. YY. Cui, Yan. “Effects of Sirtuin 1 on the proliferation and osteoblastic differentiation of periodontal ligament stem cells and stem cells from apical papilla.” Genet Mol Res ;15(1). doi: 10.4238/gmr.15015234 2016.
[5] Y. Huang, J. Huo, FQ. Liu, J. Liu, XJ. Zhang, CH. Guo, LH. Song. “Resveratrol Promotes in vitro Differentiation of Osteoblastic MC3T3-E1 Cells via Potentiation of the Calcineurin/NFATc1 Signaling Pathway.” Biochemistry (Mosc) ;84(6):686-692 2019.
[6] M. Zhao, SY. Ko, IR. Garrett, GR. Mundy, GE. Gutierrez, JR. Edwards. “The polyphenol resveratrol promotes skeletal growth in mice through a sirtuin 1-bone morphogenic protein 2 longevity axis.” Br J Pharmacol ;175(21):4183-4192 2018.
[7] BL. Furman. “Streptozotocin-Induced Diabetic Models in Mice and Rats”. Curr Protoc Pharmacol;70:5.47.1-20 2015.