The aim of this study was to investigate how melatonin administration  diabetic elderly female  rats affects the levels of bone tissue SIRT1. The study was performed on female aged rats (16 months old) who were provided by The Experimental Medicine Research and Application Center of Selçuk University.  A total of 24 elderly female rats were divided into 4 groups: Group 1. Control, Group 2. Control + Melatonin, Group 3. Diabetes, Group 4. Diabetes + Melatonin. In order to induce diabetes, the animals were intraperitoneally (IP) injected with 40 mg/kg streptozotocin (STZ). The animals were supplemented with 5 mg/kg/day melatonin IP for 4 weeks.  At the end of the study, SIRT1 protein gene expression  were determined by PCR, on bone tissue samples obtained from the  animals  sacrificed  under general anesthesia. In our study, the highest bone tissue SIRT1 expression values were obtained in the diabetes + melatonin group (G4) and the lowest bone SIRT 1 levels were found in the diabetes group (G3). Our results suggest that melatonin supplementation increases bone SIRT1 expression in diabetic elderly female rats.

Keywords - old female rats, diabetes mellitus, bone tissue, melatonin, SIRT1

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